Objective : This study was undertaken to determine which compound of Bee Venom for herb-acupuncture has cytotoxicity on mouse mast cell line.
Methods : We compared crude bee venom and its compounds such as melittin, mast cell degranulating peptide (MCD peptide), apamin with control groups on cytotoxicity by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
Results & Conclusion : 1. Crude bee venom showed significant cytotoxic effect(p<0.01) in 1 hour treatment with 1㎍/㎖ in comparison with control group in 1 hour treatment with low concentration of 10-4㎍/㎖, 10-3㎍/㎖, 10-2㎍/㎖, 10-l㎍/㎖ and l㎍/㎖, but it showed no significant cytotoxic effect in 6 hours treatment.
2. Melittin group showed no significant cytotoxic effect in comparison with control group in I and 6 hours treatment with low concentration of 10-4㎍/㎖, 10-3㎍/㎖, 10-2,㎍/㎖, 10-l㎍/㎖ and l/㎍/㎖.
3. MCD peptide and Apamin group showed no significant cytotoxic effect in comparison with control group in I and 6 hours treatment with low concentration of 10-4㎍/㎖ 10-3㎍/㎖, 10-2㎍/㎖, 10-l㎍/㎖ and l㎍/㎖
4. Crude bee venom showed significant cytotoxic effect(p<0.01) in I and 6 hours treatment in comparison with control group in I and 6 hours treatment with high concentration of 10㎍/㎖, 20u㎍/㎖ and 102㎍/㎖
5. Melittin group showed significant cytotoxic efect(p<0.01) in 1 hour treatment in comparison with control group in 1 hour treatment with high concentration of 10㎍/㎖ but it showed no significant cytotoxic effect in 6 hours treatment.
6. Crude bee venom and its compounds have more cytotoxic effect in 1 hour treatment than in 6 hours treatment. It means cytotoxicity tends to decrease according to the treatment time.
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